Buffer Stability
| Drug candidates can be instable in various physiologically-relevant buffers, such as simulated interstinal fluid or simulated gastric fluid. The stability in SIF or SGF may provide insightful information for understanding the PK behavior of the compounds. | ||
Buffer Stability Assays: Standard Assay Conditions (Customizable) |
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Compound Concentration
20 µM
Time Points
Time points (0, 15, 30, 60 minutes) for half-life determinations; zero and 60 minutes for %disappearance of parent compound
Detection Method
LC-MS/MS
Control compounds
Omeprazole
Buffers
Fasted or fed simulated intestinal/gastric fluids, with and without enyzmes pancreatin/pepsin
Deliverables
%disappearance of parent compound, Half-life (t1/2)
Turnaround Time
2-3 Days
Compound requirements
20 μL 10 mM DMSO stock