Hepatocyte Stability

////Hepatocyte Stability
Hepatocyte Stability 2018-05-15T15:16:58+00:00

 

Hepatocyte Stability

Hepatocytes are a living system, which closely mimics the in vivo situation. They contain all the liver metabolizing enzymes and their cofactors. It is a preferred system for in vitro drug metabolism studies.

Hepatocyte Stability Assays: Standard Assay Conditions (Customizable)

Compound Concentration
1 µM

Hepatocyte density
500,000 cells/mL

Time Points
Time points (0, 60, 120, 180 minutes) for half-life and intrinsic clearance determinations; zero and 60 minutes for %disappearance of parent compound

Detection Method
LC-MS/MS

Control compounds
Midazolam

Species
Human, rat, mouse, dog and monkey (additional species are available on request)

Deliverables
%disappearance of parent compound, Half-life (t1/2) and intrinsic clearance (CLint)

Turnaround Time
2-3 Days

Compound requirements
20 μL 10 mM DMSO stock