Plasma/Blood Stability
| Plasma and blood contain esterases and proteases activities. Compounds with ester and amide bonds, such as many pro-drugs and peptide-based compounds, should be routinely tested for plasma/blood stability. | ||
Plasma/Blood Stability Assays: Standard Assay Conditions (Customizable) |
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Compound Concentration
1 µM
Time Points
Time points (0, 60, 120, 240 minutes) for half-life determinations; zero and 240 minutes for %disappearance of parent compound
Detection Method
LC-MS/MS
Control compounds
Verapamil and Propantheline
Species
Human, rat, mouse, dog and monkey (additional species are available on request)
Deliverables
%disappearance of parent compound, Half-life (t1/2)
Turnaround Time
2-3 Days
Compound requirements
20 μL 10 mM DMSO stock