S9 Stability

S9 Stability 2018-05-15T15:18:09+00:00

 

S9 Stability

S9 contains all the enzymes present in the microsomes and cytosol, such as aldehyde oxidase and many enzymes responsible for phase II metabolism.

S9 Stability Assays: Standard Assay Conditions (Customizable)

Compound Concentration
1 µM

S9 Concentration
2 mg protein/mL

Time Points
Time points (0, 15, 30, 60 minutes) for half-life and intrinsic clearance determinations; zero and 30 minutes for %disappearance of parent compound

Detection Method
LC-MS/MS

Control compounds
Verapamil

Species
Human, rat, mouse, dog and monkey (additional species are available on request)

Deliverables
%disappearance of parent compound, Half-life (t1/2) and intrinsic clearance (CLint)

Turnaround Time
2-3 Days

Compound requirements
20 μL 10 mM DMSO stock