|The Caco-2 system has become the standard format to relate drug permeability to oral drug absorption. The Caco-2 cell model exhibits morphological as well as functional similarities to human intestinal enterocytes.
Features of Caco-2 cell monolayers:
Caco-2 permeability assays could be useful to predict
Quintara Discovery offers a validated Caco-2 permeability assay to assess transport in both directions, apical to basolateral (A-B) and basolateral to apical (B-A), across the cell monolayer. The bi-directional format of the assay allows to understand any involvement of a transport systems on the efflux of a test compound.
Caco-2 Permeability: Standard Assay Conditions (Customizable)
Testing Compound Concentration
HBSS, pH 7.4 (or pH 6.5) on apical side; HBSS, pH 7.4 on basolateral side, 1-3% BSA optional on the receiving side
24-well or 96-well Transwell plates
1 hour at 37C
Papp value for A-B and B-A directions, efflux ratio
20 μL 10 mM DMSO stock