Quintara Discovery offers dedicated assay development and screening services by a talented assay team having direct experience with >200 drug targets and HTS campaigns. We develop high-fidelity biochemical, cellular, PBMC and whole blood based assays, optimize assays in various formats for weekly screening or HTS campaign, execute screening, and deliver robust results.We provide expertise on a variety of target classes, including kinases, metabolic enzymes, GPCRs, transporters, membrane receptors and epigenetic targets. Using our state-of-the-art instrumentation we can provide a variety of assays formats and readouts such as ELISA, chemiluminescence, fluorescence intensity, fluorescence polarization, fluorescence resonance energy transfer (FRET), and label-free detection by mass spectrometry.

Assay Formats:

  • Biochemical (cell-free systems) assay for compound-target interactions
  • Simple inhibition (IC50)
  • Binding competition
  • Reversibility, off-rates
  • Cell-based Assay
  • Reporter Assays using cell-lines
  • Assay using Primary cells (PBMCs/whole blood)

Mechanism of Action Studies

Quintara Discovery also provides assay to address mechanism of inhibition by drug molecules including reversibility, off-rates, duration of action, and mode of inhibition (competitive/non-competitive/un-competitive).