ELISA Assay Development

Typically at the beginning of an ELISA project, our scientists will discuss with clients for assay formats and reagent availability. Clients can provide antibodies or antigens, or we identify and obtain potential antibodies and antigens specific for the target. A typical assay development process includes the following steps:

  • Characterize the specificity of the antibodies and antigens using Western blot.
  • Determine optimal dilutions for all antibodies and antigens
  • Determine the throughput: Assays can be developed in 96w or 384w format
  • Optimize incubation times, temperatures and buffers
  • Optimize the assay for maximum sensitivity and precision
  • Determine lower and upper limits of detection
  • Optimize standards and standard curves for quantitation