Tissue Protein Binding

////Tissue Protein Binding
Tissue Protein Binding 2018-05-15T16:12:38+00:00

 

Tissue Protein Binding

The binding of drug to tissue (such as rat brain or inactivated hepatocyte) proteins is a major determinant of drug disposition and plays an important effect on pharmacodynamics since only the free (unbound) drug is responsible for the pharmacological activity.

Assay Methodology

At Quintara Discovery, tissue protein binding for a test compound is determined using the Rapid Equilibrium Dialysis (RED) method performed in a 48-well format.

Tissue Protein Binding: Standard Assay Conditions (Customizable)

Testing Compound Concentration
1 μM

Dialysis buffer
PBS, pH 7.4

Incubation Period
4 hour at 37 °C, 5% or 10% CO2 if needed

Detection Method
LC-MS/MS

Control Compound
Verapamil

Species
Human, rat, mouse, dog and monkey (additional species are available on request)

Deliverables
% Bound

Turnaround Time
2-3 Days

Compound requirements
20 μL 10 mM DMSO stock