|The Madin-Darby canine kidney (MDCK) cell model is one of the commonly used cell monolayer systems to assess the human intestine barrier.
Advantages of MDCK (Madin-Darby Canine Kidney) Cells
The MDCK permeability screen (uni-directional) is performed to measure test compounds across a monolayer of MDCK cells. The cells seeded on 24- or 96-well plates to form a confluent monolayer are used to determine the efflux of the test compound when added to the apical side of the membrane. The transport of the compound across the monolayer is monitored over a 60 min time period.
MDCK Permeability: Standard Assay Conditions (Customizable)
Testing Compound Concentration
HBSS, pH 7.4
24-well or 96-well Transwell plates
1 hour at 37C
Atenolol and propranolol
Papp value for A-B direction
20 μL 10 mM DMSO stock