MDCK-MDR1 Permeability

This assay model allows for an in vitro approach for evaluating possible interactions with the efflux protein Pgp, by monitoring the permeability of a known substrate, using the MDCK-MDR1 cell line in the presence of the study compound.

Assay Methodology

The efflux ratios can be determined in the MDCK-MDR1 cell line, by measuring the apparent permeability (Papp) values of test compound for both the apical to basal (A>B) and basal to apical (B>A).

MDCK-MDR1 Permeability: Standard Assay Conditions (Customizable)

Testing Compound Concentration
10 μM

Assay Buffer
HBSS, pH 7.4

Plate Format
24-well or 96-well Transwell plates

Transport Period
1 hour at 37C

Detection Method

Control Compounds
Digoxin and propranolol

Papp value for A-B and B-A directions, efflux ratio

Turnaround Time
5-7 Days

Compound requirements
20 μL 10 mM DMSO stock