Tissue Protein Binding
|The binding of drug to tissue (such as rat brain or inactivated hepatocyte) proteins is a major determinant of drug disposition and plays an important effect on pharmacodynamics since only the free (unbound) drug is responsible for the pharmacological activity.
At Quintara Discovery, tissue protein binding for a test compound is determined using the Rapid Equilibrium Dialysis (RED) method performed in a 48-well format.
Tissue Protein Binding: Standard Assay Conditions (Customizable)
Testing Compound Concentration
PBS, pH 7.4
4 hour at 37 °C, 5% or 10% CO2 if needed
Human, rat, mouse, dog and monkey (additional species are available on request)
20 μL 10 mM DMSO stock