MDCK-MDR1 Permeability
| This assay model allows for an in vitro approach for evaluating possible interactions with the efflux protein Pgp, by monitoring the permeability of a known substrate, using the MDCK-MDR1 cell line in the presence of the study compound. | ||
Assay MethodologyThe efflux ratios can be determined in the MDCK-MDR1 cell line, by measuring the apparent permeability (Papp) values of test compound for both the apical to basal (A>B) and basal to apical (B>A). |
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MDCK-MDR1 Permeability: Standard Assay Conditions (Customizable) |
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Testing Compound Concentration
10 μM
Assay Buffer
HBSS, pH 7.4
Plate Format
24-well or 96-well Transwell plates
Transport Period
1 hour at 37C
Detection Method
LC-MS/MS
Control Compounds
Digoxin and propranolol
Deliverables
Papp value for A-B and B-A directions, efflux ratio
Turnaround Time
5-7 Days
Compound requirements
20 μL 10 mM DMSO stock