Hepatocyte Stability
| Hepatocytes are a living system, which closely mimics the in vivo situation. They contain all the liver metabolizing enzymes and their cofactors. It is a preferred system for in vitro drug metabolism studies. | ||
Hepatocyte Stability Assays: Standard Assay Conditions (Customizable) |
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Compound Concentration
1 µM
Hepatocyte density
500,000 cells/mL
Time Points
Time points (0, 60, 120, 180 minutes) for half-life and intrinsic clearance determinations; zero and 60 minutes for %disappearance of parent compound
Detection Method
LC-MS/MS
Control compounds
Midazolam
Species
Human, rat, mouse, dog and monkey (additional species are available on request)
Deliverables
%disappearance of parent compound, Half-life (t1/2) and intrinsic clearance (CLint)
Turnaround Time
2-3 Days
Compound requirements
20 μL 10 mM DMSO stock