Microsomal Stability
| Liver microsomes are the most frequently used form of tissue preparations for in vitro drug metabolism studies. Pooled microsomes contain all active CYP450 enzymes, which are responsible for the metabolism of the majority of drugs, and other enzymes such as FMO and UGTs. Quintara Discovery offers a broad microsomal collection and has a capacity for assaying hundreds of compounds on a weekly basis. | ||
Microsomal Stability Assays: Standard Assay Conditions (Customizable) |
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Compound Concentration
1 µM
Microsome Concentration
0.5 mg protein/mL
Time Points
Time points (0, 15, 30, 60 minutes) for half-life and intrinsic clearance determinations; zero and 30 minutes for %disappearance of parent compound
Detection Method
LC-MS/MS
Control compounds
Verapamil
Species
Human, rat, mouse, dog and monkey (additional species are available on request)
Deliverables
%disappearance of parent compound, Half-life (t1/2) and intrinsic clearance (CLint)
Turnaround Time
2-3 Days
Compound requirements
20 μL 10 mM DMSO stock