S9 Stability
| S9 contains all the enzymes present in the microsomes and cytosol, such as aldehyde oxidase and many enzymes responsible for phase II metabolism. | ||
S9 Stability Assays: Standard Assay Conditions (Customizable) |
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Compound Concentration
1 µM
S9 Concentration
2 mg protein/mL
Time Points
Time points (0, 15, 30, 60 minutes) for half-life and intrinsic clearance determinations; zero and 30 minutes for %disappearance of parent compound
Detection Method
LC-MS/MS
Control compounds
Verapamil
Species
Human, rat, mouse, dog and monkey (additional species are available on request)
Deliverables
%disappearance of parent compound, Half-life (t1/2) and intrinsic clearance (CLint)
Turnaround Time
2-3 Days
Compound requirements
20 μL 10 mM DMSO stock